Paper on R-Loop formation and structure published in PNAS

Our latest research on “Protein-mediated stabilization and nicking of the nontemplate DNA strand dramatically affect R-loop formation in vitro” has been published in the prestigious journal PNAS, with colleagues from the University of California, Davis; Ethan Holleman, Tadas Sereiva, Stella Hartono and Frederic Chedin. This work demonstrates how nicking the non-template strand before transcription can significantly increase the formation of three-strand nucleic acid structures known as R-Loops. Our high-resolution Atomic Force Microscopy revealed that nicks change the morphology of the R-Loop, allowing the formation of previously unreported forked structures.

You can read the press release from the Royce team here: Royce Linkedin Post

and you can read the full paper here: https://doi.org/10.1073/pnas.2509309122