PUBLICATIONS
Multiscale topological analysis of kinetoplast DNA via high-resolution AFM.
B. Diggines, S. Whittle, I. Yadav, E. P. Holmes, D. E. Rollins, T. E. Catley, P. S. Doyle, A. L. B. Pyne, Phys. Chem. Chem. Phys. 26, 25798–25807 (2024).
Complement-mediated killing of Escherichia coli by mechanical destabilization of the cell envelope
G. Benn, C. Bortolini, D. M. Roberts, A. L. B. Pyne, S. Holden, B. W. Hoogenboom, EMBO J., (2024)
Lipidated DNA Nanostructures Target and Rupture Bacterial Membranes
I. D. Bennett, J. R. Burns, M. G. Ryadnov, S. Howorka, A. L. B. Pyne, Small, 20 2207585 (2024)
Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription.
Á. Dos Santos, D. E. Rollins, Y. Hari-Gupta, H. McArthur, M. Du, S. Y. Z. Ru, K. Pidlisna, A. Stranger, F. Lorgat, D. Lambert, I. Brown, K. Howland, J. Aaron, L. Wang, P. J. I. Ellis, T.-L. Chew, M. Martin-Fernandez, A. L. B. Pyne, C. P. Toseland, Nat Commun 14, 2855 (2023).
Atomic Force Microscopy of DNA and DNA-Protein Interactions
P. J. Haynes, K. H. S. Main, B. Akpinar, A. L. B. Pyne, Chromosome Architecture, in Methods in Molecular Biology, 2476, 43–62 (2022)
Atomic force microscopy—A tool for structural and translational DNA research
K. H. S. Main, J. I. Provan, P. J. Haynes, G. Wells, J. A. Hartley, A.L.B. Pyne, APL Bioeng. 5(3), 031504 (2021).
TopoStats – A program for automated tracing of biomolecules from AFM images.
J. G. Beton, R. Moorehead, L. Helfmann, R. Gray, B. W. Hoogenboom, A. P. Joseph, M. Topf, A. L. B. Pyne, Methods 193, 68–79 (2021).
Base-pair resolution analysis of the effect of supercoiling on DNA flexibility and major groove recognition by triplex-forming oligonucleotides.
A. L. B. Pyne, A. Noy , K. H. S. Main, V. Velasco-Berrelleza, M. M. Piperakis, L. A. Mitchenall, F. M. Cugliandolo, J. G. Beton, C. E. M. Stevenson, B. W. Hoogenboom, A. D. Bates, A. Maxwell, S. A. Harris, Nat Commun 12, 1053 (2021).
Cantilever Sensors for Rapid Optical Antimicrobial Sensitivity Testing.
I. Bennett, A. L. B. Pyne, R. A. McKendry, ACS Sens. 5, 3133–3139 (2020).
Single-molecule kinetics of pore assembly by the membrane attack complex.
E. S. Parsons, G. J. Stanley, A. L. B. Pyne, A. W. Hodel, A. P. Nievergelt, A. Menny, A. R. Yon, A. Rowley, R. P. Richter, G. E. Fantner, D. Bubeck, B. W. Hoogenboom, Nat Commun 10, 2066 (2019).
PEGylated surfaces for the study of DNA–protein interactions by atomic force microscopy.
B. Akpinar, P. J. Haynes, N. A. W. Bell, K. Brunner, A. L. B. Pyne, B. W. Hoogenboom, Nanoscale 11, 20072–20080 (2019).
Imaging live bacteria at the nanoscale: comparison of immobilisation strategies.
G. Benn, A. L. B. Pyne, M. G. Ryadnov, B. W. Hoogenboom, Analyst 144, 6944–6952 (2019).
Bacterial killing by complement requires membrane attack complex formation via surface-bound C5 convertases.
D. A. Heesterbeek, B. W. Bardoel, E. S. Parsons, I. Bennett, M. Ruyken, D. J. Doorduijn, R. D. Gorham, E. T. Berends, A. L. Pyne, B. W. Hoogenboom, S. H. Rooijakkers, The EMBO Journal 38, e99852 (2019).
Tuneable poration: host defense peptides as sequence probes for antimicrobial mechanisms.
M.-P. Pfeil, A. L. B. Pyne, V. Losasso, J. Ravi, B. Lamarre, N. Faruqui, H. Alkassem, K. Hammond, P. J. Judge, M. Winn, G. J. Martyna, J. Crain, A. Watts, B. W. Hoogenboom, M. G. Ryadnov, Sci Rep 8, 14926 (2018).
DNA Origami Inside-Out Viruses.
J. R. Burns, B. Lamarre, A. L. B. Pyne, J. E. Noble, M. G. Ryadnov, ACS Synth. Biol. 7, 767–773 (2018).
Engineering monolayer poration for rapid exfoliation of microbial membranes.
A. Pyne, M.-P. Pfeil, I. Bennett, J. Ravi, P. Iavicoli, B. Lamarre, A. Roethke, S. Ray, H. Jiang, A. Bella, B. Reisinger, D. Yin, B. Little, J. C. Muñoz-García, E. Cerasoli, P. J. Judge, N. Faruqui, L. Calzolai, A. Henrion, G. J. Martyna, C. R. M. Grovenor, J. Crain, B. W. Hoogenboom, A. Watts, M. G. Ryadnov, Chem. Sci. 8, 1105–1115 (2017).
Studies of G-quadruplexes formed within self-assembled DNA mini-circles.
B. Klejevskaja, A. L. B. Pyne, M. Reynolds, A. Shivalingam, R. Thorogate, B. W. Hoogenboom, L. Ying, R. Vilar, Chem. Commun. 52, 12454–12457 (2016).
Biomimetic Hybrid Nanocontainers with Selective Permeability.
L. Messager, J. R. Burns, J. Kim, D. Cecchin, J. Hindley, A. L. B. Pyne, J. Gaitzsch, G. Battaglia, S. Howorka, Angewandte Chemie 128, 11272–11275 (2016).
Imaging DNA Structure by Atomic Force Microscopy
A. L. B. Pyne, B. W. Hoogenboom, in Chromosome Architecture, M. C. Leake, Ed. (Springer New York, New York, NY, 2016; vol. 1431 of Methods in Molecular Biology, pp. 47–60.
Single-Molecule Reconstruction of Oligonucleotide Secondary Structure by Atomic Force Microscopy.
A. Pyne, R. Thompson, C. Leung, D. Roy, B. W. Hoogenboom, Small 10, 3257–3261 (2014).
Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers.
P. D. Rakowska, H. Jiang, S. Ray, A. Pyne, B. Lamarre, M. Carr, P. J. Judge, J. Ravi, U. I. M. Gerling, B. Koksch, G. J. Martyna, B. W. Hoogenboom, A. Watts, J. Crain, C. R. M. Grovenor, M. G. Ryadnov, Proc. Natl. Acad. Sci. U.S.A. 110, 8918–8923 (2013).
Atomic Force Microscopy with Nanoscale Cantilevers Resolves Different Structural Conformations of the DNA Double Helix.
C. Leung, A. Bestembayeva, R. Thorogate, J. Stinson, A. Pyne, C. Marcovich, J. Yang, U. Drechsler, M. Despont, T. Jankowski, M. Tschöpe, B. W. Hoogenboom, Nano Lett. 12, 3846–3850 (2012).
High-speed atomic force microscopy of dental enamel dissolution in citric acid.
A. Pyne, W. Marks, L. M. Picco, P. G. Dunton, A. Ulcinas, M. E. Barbour, S. B. Jones, J. Gimzewski, M. J. Miles, Arch. Histol. Cytol. 72, 209–215 (2009).
* authors contributed equally to this work