New Nature Paper on CRISPR/Cas9!

We are thrilled to share our latest collaborative research with Imperial College London and the MRC Laboratory of Medical Sciences (LMS), recently published in Nature. The study reveals that DNA supercoiling of the molecule is a critical factor in the accuracy of CRISPR-Cas9 gene editing. By utilizing our lab’s high-resolution AFM to visualize DNA minicircles, we were able to demonstrate how the mechanical stress of "underwound" DNA can lead to off-target binding. The fantastic images were collected by Eddie Rollins and the analysis was performed by Sylvia Whittle. This complements the stunning cryoEM data obtained by Quentin Smith from David Rueda’s lab. These insights provide a new framework for improving the safety and precision of gene therapies.

You can read the full press release from Imperial College here and the full article here.